Research Article

Rhubarb Tannins Extract Inhibits the Expression of Aquaporins 2 and 3 in Magnesium Sulphate-Induced Diarrhoea Model

Figure 3

Rhubarb tannins extract (RTE) reduces the protein expression of aquaporins (AQPs) 2 and 3 in the colons of magnesium sulphate-induced diarrhoea mice by fluorescent immunohistochemistry. ((a) and (b)) Localization of AQPs 2 and 3 in both the apical and lateral mucosal epithelial cells in the proximal colons of normal control, vehicle-treated diarrhea, and RTE- (500 mg/kg) treated diarrhoea mice. Negative controls in which the AQPs 2 and 3 antibodies were replaced with normal rabbit immunoglobulin (IgG) at identical concentrations resulted in a lack of specific staining. Tissue is colocalized with DAPI to demonstrate the location of cell nuclei (blue). The images in merge’ panel are the big magnification in merge panel, respectively. ((c) and (d)) Positive expression levels of AQPs 2 and 3 in the colons of normal control, vehicle-treated diarrhea, and RTE- (125, 250, and 500 mg/kg) treated diarrhoea mice. 10 microscopic fields were selected randomly and AQPs 2 and 3 positive cells were counted. The AQPs 2 and 3 positive expression levels of the control were taken as 100%. Data are represented as the mean ± SD. ### versus normal control group. , , and versus vehicle group, respectively. in each group and each assay was repeated 3 times.
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