Characterization of Uptake and Internalization of Exosomes by Bladder Cancer Cells
Exosome uptake in bladder cancer cells is dose dependent. Labeled exosomes isolated from SW780 cells were incubated at various concentrations with SW780 cells for 4 hours. This experiment was performed at least three times, with data from a representative experiment shown. A minimum of 10,000 cells were analyzed for each sample. (a) Representative images from the Amnis ImageStreamX. (b) Spot count data using the IDEAS software. (c) Fluorescence intensity of labeled exosomes, quantitated by the IDEAS software. (d) Max pixel quantitation of labeled exosomes. (e) Median pixel quantitation of labeled exosomes. Data is represented as average ± SEM.