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BioMed Research International
Volume 2014, Article ID 625695, 8 pages
Research Article

Differential Expression of Cholecystokinin A Receptor in Gallbladder Cancer in the Young and Elderly Suggests Two Subsets of the Same Disease?

1Department of Surgical Gastroenterology, King George’s Medical University, Lucknow 226003, India
2Developmental Toxicology Division, Indian Institute of Toxicology Research, Lucknow 226003, India
3Department of Biochemistry, King George’s Medical University, Lucknow 226003, India
4Department of Biochemistry, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh 462024, India
5All India Institute of Medical Sciences, Bhopal, Madhya Pradesh 462024, India

Received 28 February 2014; Revised 27 May 2014; Accepted 27 May 2014; Published 15 June 2014

Academic Editor: Saulius Butenas

Copyright © 2014 Hasan Raza Kazmi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Cholecystokinin type A receptor (CCKAR) is known to be overexpressed in variety of human malignancies but information regarding its expression in gallbladder cancer (GBC) is limited. Attempts were now made to investigate expression pattern of CCKAR mRNA and protein in controls and GBC patients and correlate it with various clinicopathological parameters following surgical resection. Materials and Methods. Gallbladder tissue samples from 64 subjects (GBC: 39; control: 25) were studied. Expression of CCKAR mRNA was evaluated by reverse transcriptase-polymerase chain reaction and confirmed using real-time polymerase chain reaction. Protein expression was studied by enzyme-linked immunosorbent assay. Results. Significantly higher expression of CCKAR mRNA and protein was observed in GBC tissues. Overexpression was also observed for stage III and in moderately and poorly differentiated tumors. When the clinicopathological parameters were compared, we found age dependent decrease in CCKAR expression. Relatively higher expression of CCKAR was observed in younger patients (age < 45 years) having more aggressive disease when compared with elderly ones (age ≥ 45 years). Conclusions. Age related differential expression of CCKAR in GBC may suggest two possible variants of the disease in this endemic belt.