Research Article

Viola tricolor Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane

Figure 5

Effects of ethyl acetate (EtOAc) fraction of Viola tricolor on proapoptotic (Bax, caspase-3) and antiapoptotic (Bcl-2) proteins expression in Neuro2a cells (a). The cell lysates were immunoprecipitated with anti-Bax, anti-Bcl-2 and anti-cleaved caspase-3 antibodies and then immunoblotted using horse radish peroxidase-conjugated goat anti-rabbit secondary antibody with β-actin as a protein loading control. Data are presented as the fold induction over control cells ((b)-(c)). , versus untreated control cells.
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(a)
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(b)
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(c)