Figure 6: Quantitation and confocal analysis of the nonphagocytic and phagocytic subpopulations of E. dispar and E. histolytica. Graph showing the percentage of nonphagocytic amoebas after different times of interaction for E. dispar (blue) and E. histolytica (red) (a). Experiments were done in triplicate for each of the analyzed times. (b) shows representative images of phagocytic and nonphagocytic subpopulations for E. dispar (left side) and E. histolytica (right side). Upper panels, light microscopy; lower panels, confocal images. Arrow head: a nonphagocytic trophozoite.