Figure 4: 13C-MFA of simplified TCA model. Fluxes were calculated using OpenMebius, OpenFLUX, and 13CFLUX. Dotted lines indicate reactions whose metabolic fluxes are predetermined. Solid line arrows: intracellular metabolic reactions. Dotted line arrows: substrate consumption and biomass synthesis. Suffix: _B, biomass drain; _EX, exo-metabolites. Metabolites: PYR, pyruvate; ACCOA, acetyl-CoA; IsoCit, isocitrate; KG, -ketoglutarate; SUC, succinate; OAA, oxaloacetate; GLU, glutamate; LYS, lysine; VAL, valine.