Activation of the AT1R/HIF-1α/ACE Axis Mediates Angiotensin II-Induced VEGF Synthesis in Mesenchymal Stem Cells

Figure 1

Upregulation of ACE after Ang II-induced VEGF synthesis. (a) Western blot analysis of ACE. MSCs were exposed to Ang II (100 nM) for 6 h, 12 h, or 24 h. , . (b) Determination of ACE activity. MSCs were exposed to Ang II (100 nM) for 1 h, 6 h, 12 h, 18 h, or 24 h. , every other group versus control. (c) Real-time qPCR examination of ACE mRNA levels. MSCs were exposed to Ang II (100 nM) for 12 h. , . (d) Real-time qPCR analysis of VEGF mRNA level, . (e) ELISA of VEGF secretion. MSCs were pretreated with or without captopril (1 μM for 1 h) before exposure to Ang II (100 nM) for 12 h. , .

(a)

(b)

(c)

(d)

(e)