Individual score of the skin reaction on challenge sites.
Group
Animal no.
Treatment
Grading scale*
Induction
Challenge
24 hr**
48 hr**
Intradermal injection
Topical application
2
6
Fish collagen (0.1%)
Fish collagen (0.1%)
Fish collagen (0.1%)
0
0
Water for injection
0
0
7
Fish collagen (0.1%)
Fish collagen (0.1%)
Fish collagen (0.1%)
0
0
Water for injection
0
0
8
Fish collagen (0.1%)
Fish collagen (0.1%)
Fish collagen (0.1%)
0
0
Water for injection
0
0
9
Fish collagen (0.1%)
Fish collagen (0.1%)
Fish collagen (0.1%)
0
0
Water for injection
0
0
10
Fish collagen (0.1%)
Fish collagen (0.1%)
Fish collagen (0.1%)
0
0
Water for injection
0
0
Notes: *Grading scale. Patch test reaction: 0: no reaction, 1: discrete or porphyritic erythema, 2: moderately fused erythema, 3: extremely severe erythema and swelling.
**Time (hours) after challenge.
