Effect of Acute Gamma Irradiation on Curcuma alismatifolia Varieties and Detection of DNA Polymorphism through SSR Marker

Table 11

SSR primers, number of alleles, product size, expected heterozygosity, observed heterozygosity, number of effective alleles, Nei's gene diversity, Shannon’s Index, and PIC.

Locus	Primer sequence (5′–3′)	Repeat motif	Dose (Gy)	𝑁𝑎	Range size (bp)	𝑇𝑚 (°C)	𝑇𝑎 (°C)	𝐻𝑒	𝐻𝑜	Ne	ℎ	𝐼	PIC
Clon01	F: ACTGGACTGTCCGAGAGCAT; R: TCGTTTAGCGACAACGGATT	(TA)6TTG(TC)16	0	4	194–212	60.7	68–58	0.78	0.00	4.00	0.75	1.38	0.71
			10	5	194–212	60.7	68–58	0.78	0.00	4.25	0.76	1.51	0.72
			20	6	194–216	60.7	68–58	0.81	0.04	4.80	0.78	1.64	0.73
Clon04	F: TAAATTTGCGAAGGCAATCC; R: CCGCAGAGGAATTTGAAGAG	(TATAG)2(AG)17	0	2	179–200	58.3	65–55	0.22	0.25	1.28	0.21	0.37	0.19
			10	3	179–200	58.3	65–55	0.28	0.24	1.38	0.27	0.52	0.25
			20	6	170–200	58.3	65–55	0.58	0.21	2.31	0.56	1.21	0.54
Clon08	F: CCGGTGAGGGTGATATCTTG; R: AAGCTCAAGCTCAAGCCAAT	(GT)10	0	4	245–274	60.7	68–58	0.67	0.75	2.91	0.65	1.21	0.61
			10	7	231–274	60.7	68–58	0.79	0.56	4.57	0.78	1.67	0.75
			20	7	245–274	60.7	68–58	0.79	0.39	4.42	0.78	1.65	0.75
Clon09	F: GGAGGAGGCAGTTGATTTGT; R: GCTTTGGTGGCTAGAGATGC	(AC)14	0	2	182–188	60.4	67–57	0.38	0.00	1.60	0.37	0.56	0.31
			10	3	182–200	60.4	67–57	0.40	0.04	1.65	0.39	0.64	0.33
			20	3	182–195	60.4	67–57	0.51	0.00	1.99	0.50	0.84	0.43
Clon10	F: GTGGGAATTGGATTGCTCTC; R: GAGAACTCCCCATGCTTCAG	(GT)7	0	4	204–232	60.7	68–58	0.67	0.25	2.91	0.65	1.21	0.61
			10	5	200–232	60.7	68–58	0.66	0.20	2.86	0.65	1.27	0.61
			20	6	200–232	60.7	68–58	0.71	0.26	3.38	0.71	1.40	0.65
Clon11	F: GGGCTTTGTTTAGTTGTCGTG; R: CAGGAATGAAGTCGGCAAC	(AGA)8	0	2	160–167	60.7	68–58	0.51	0.00	2.00	0.50	0.69	0.37
			10	4	163–182	60.7	68–58	0.64	0.00	2.72	0.63	1.13	0.56
			20	5	163–182	60.7	68–58	0.62	0.00	2.58	0.61	1.12	0.54
Clon12	F: GATTGGATCACATGGTGTGC; R: TGGGTTGATGGTTTCTCTGTT	(CT)20	0	5	195–231	59.0	66–76	0.71	0.50	3.20	0.68	1.38	0.65
			10	6	195–231	59.0	66–76	0.73	0.28	3.52	0.71	1.45	0.67
			20	7	195–255	59.0	66–76	0.78	0.56	4.39	0.77	1.70	0.75
Clon14	F: TCAGTCGAGGGGTTCCTACT; R: GAGAGCTGATCGCAAAAACC	(CTT)7	0	2	175–181	60.7	68–58	0.38	0.00	1.60	0.37	0.57	0.31
			10	3	171–181	60.7	68–58	0.55	0.00	2.21	0.54	0.91	0.47
			20	3	171–181	60.7	68–58	0.51	0.00	2.1	0.51	0.81	0.42
Total			0	25
			10	36
			20	41
Mean			0	3.1				0.5±0.1	0.2±0.2	2.5±0.9	0.52±0.1	0.9±0.4	0.47
			10	4.5				0.6±0.1	0.1±0.1	2.9±1.1	0.60±0.1	1.1±0.4	0.54
			20	5.1				0.6±0.1	0.2±0.2	2.9±1.2	0.61±0.1	1.2±0.4	0.61

na: number of alleles 𝐻_𝑜: observed heterozygosity; 𝐻_𝑒: expected heterozygosity; Ne: effective number of alleles; ℎ: Nei’s gene diversity; 𝐼: Shannon’s index; PIC: polymorphic information content.