Detection of Newcastle Disease Virus Minor Genetic Variants by Modified Single-Stranded Conformational Polymorphism Analysis

Table 1

List of NDV strains with different types of templates for PCR or RT-PCR and highlighted point mutations affecting their virulence.

Strain	Templates*	GenBank acc. number	Nucleotide motif**	ICPI	Pathotype
La Sota	nR, aR, nD	AF077761	GGGAGACAGGGGCGCCTT	0.4 [1]	Lentogenic
Roakin	nR, aR, nD	AY289000	AGGAGACAGAAACGCTTT	1.45 [1]	Mesogenic
Italy	nR, aR, nD	AY562989	AGGAGGAAGAAACGCTTT	1.55 [73]	Velogenic
98-1154	aR, aD	AY935491	AGGAGACAGGGGCGTCTT	0.47 [60]	Lentogenic
99-0868-2	aR, aD	AY935496	AGGAGACAGAGGCGTCTT	1.38 [60]	Mesogenic
99-0655	aR, aD	AY935494	AGGAGACAGAGGCGTTTT	1.61 [60]	Velogenic

∗nR: genomic RNA isolated from live-virus vaccines, aR: RNA obtained by transcription of cloned fusion protein gene fragment from a plasmid, nD: plasmids with cloned cDNA from RT-PCR of genomic RNA, and aD: plasmids with cloned synthetized fusion protein gene fragment.
∗∗nucleotide sequence for F₀ cleavage site (position 4877–4894 in La Sota genome).