A Time-Dose Model to Quantify the Antioxidant Responses of the Oxidative Hemolysis Inhibition Assay (OxHLIA) and Its Extension to Evaluate Other Hemolytic Effectors

Table 1

Description of the references used to collect data to be analyzed in this study, the figures, variable or agent involved, and some relevant conditions of the assays.

Case	Reference	Figure	Effector	Conditions	Method
A: Antioxidant effector analysis
A1	[26]	Figure 20	Trolox (0-(25)-125 M).	0.7% (v/v) in PBS (pH 7.4), 37°C, 40 mM AAPH.	OxHLIA^a
A2	[30]	Figure 3	Trolox (0-(25)-100 M).	0.7% (v/v) in PBS (pH 7.4), 37°C, 40 mM AAPH.	OxHLIA^b
A3	[36]	Figure 5	Ascorbic Acid (0-(100)-300 M).	0.7% (v/v) in PBS (pH 7.4), 37°C, 50 mM AAPH.	OxHLIA^a
A4	[37]	Figure 1(a)	CazNH (0, 6.17, 9.26, 12.3, 15.4, 20.6 M)	10 mM PBS (pH 7.4), 37°C, 40 mM AAPH.	OxHLIA^a
A5	[37]	Figure 1(b)	IsbNH (0, 8.26, 12.4, 16.5, 20.7, 24.8 M)	10 mM PBS (pH 7.4), 37°C, 40 mM AAPH.	OxHLIA^a
A6	[37]	Figure 1(c)	DpaNH (0, 3, 6, 9, 12, 15 M)	10 mM PBS (pH 7.4), 37°C, 40 mM AAPH.	OxHLIA^a
A7	[37]	Figure 1(d)	PtzNH (0, 20.3, 30.5, 37.3, 50.8, 61.0 M)	10 mM PBS (pH 7.4), 37°C, 40 mM AAPH.	OxHLIA^a
A8	[37]	Figure 1(e)	PozNH (0, 9.27, 10.8, 12.4, 13.9, 15.5 M)	10 mM PBS (pH 7.4), 37°C, 40 mM AAPH.	OxHLIA^a
B: Another effector analysis
E1	[36]	Figure 3	AAPH (0-(20)-100 M).	0.7% (v/v) in PBS (pH 7.4), 37°C.	OxHLIA^a
E2	[38]	Figure 1	DTAB surfactant (2.25, 4.5, 5.625, 6.75, 11.25%).	10 mM PBS (pH 7.4), 37°C.	Osmotic fragility test^a
E3	[39]	Figure 7	NaHCO3 (1, 1.5, 1.8, 2 mM).	pH 7.2, 20°C.	Isotonic hemolytic test^a
E4	[28]	Figure 4	Temperature (15, 20, 25, 30, 35, 40, 45°C).	10 mM PBS (pH 7.4), palytoxin (0.25 pg/L).	Palytoxin hemolytic activity^c

Test-tube format.
^bMicroplate format.
^cHalf-way between test-tube and microplate format.