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BioMed Research International
Volume 2014 (2014), Article ID 637821, 8 pages
Research Article

Effect of Thickness of HA-Coating on Microporous Silk Scaffolds Using Alternate Soaking Technology

1Institute of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China
2Department of Orthopaedics, 513 Hospital of PLA, Lanzhou 732750, China
3College of Science, Air Force Engineering University, Xi’an 710032, China
4Department of Military Medical Training, Comprehensive Training Base of Lanzhou, Hutubi 831200, China

Received 4 April 2014; Accepted 4 June 2014; Published 29 June 2014

Academic Editor: Guoxian Pei

Copyright © 2014 Hongguo Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Hydroxyapatite (HA) can be coated on various materials surface and has the function of osteogenicity. Microporous silk scaffold has excellent biocompatibility. In this study, alternate soaking technology was used to coat HA on microporous silk scaffolds. However, the cell proliferation was found to decrease with the increasing thickness (cycles of soaking) of HA-coating. This study aims to determine the best thickness (cycles of soaking) of HA-coating on microporous silk scaffolds. The SEM observation showed that group with one cycle of alternate soaking (1C-HA) has the most optimal porosity like non-HA-modified microporous silk scaffolds. The proliferation of osteoblasts has no significant difference between noncoated HA (N-HA) and 1C-HA groups, which are both significantly higher than those in two cycles of soaking (2C-HA) and three cycles of soaking (3C-HA) groups. The transcription levels of specific genes (runx2 and osteonectin) in osteoblasts of 1C-HA group were significantly higher than those of N-HA group. Moreover, the levels showed no significant difference among 1C-HA, 2C-HA, and 3C-HA groups. In conclusion, microporous silk scaffold with 1 cycle of HA-coating can combine the biocompatibility of silk and osteogenicity of HA.