Saccharin Sulfonamides as Inhibitors of Carbonic Anhydrases I, II, VII, XII, and XIII
The binding of compounds 1, 3, and 4 to CA XIII, determined by the fluorescent thermal shift assay (FTSA). Panels (a)–(c) show the protein melting fluorescence curves as a function of temperature at several added compound concentrations. Saccharin did not exhibit a shift (a) while compounds 3 (b) and 4 (c) exhibited a significant shift. Panel (d) shows the resultant three compound dosing curves, the dependencies of the protein melting temperature on the added three compound concentrations. Datapoints are the experimental values obtained from panels (a)–(c) and the solid lines are simulated according to the model as described in Materials and Methods. Experiments were performed at pH 7.0 in sodium phosphate buffer.