Research Article
Detection of Genetic Variations in Coagulopathy-Related Genes Using Ramified Rolling Circle Amplification
Table 1
Nucleotide sequences of C-probes, capture probes and primers.
| Oligo Name | Sequence | Targeted locus | Targeted allele | Targeted strand | Oligo role |
| Cpr8FVWt+ | GCCTGTCCAGGGATCTGCTCTTACAATACGAGAACACCCGATTGAG AGAGTTTGGAAGTGTAGGCGTGAAGTCCATAACACATACCTGTATTC CTC | FV 1691 G-A | Wt | + | C-probe | CPFV+ | Bio-TCAGAATTTCTGAAAGGTTACTTC-Spacer C3 | FV 1691 G-A | Wt | + | Target capture | Cpr8Fwd61_22 | ACACTTCCAAACTCTCTCAATC | FV 1691 G-A | Wt | + | RAM Fwd Primer | Cpr8Rvs03_20 | CTGTCCAGGGATCTGCTCTT | FV 1691 G-A | Wt | + | RAM Rev Primer |
| Cpr9FVMt− | AGGAATACAGGTATTTTGTCCTTGAAGTAACCCTCGTGAAAGCCCTA CTCTATGAAATCTTGTAGCAGGACTCCGTTTAGCAGCACTGGACAG GCA | FV 1691 G-A | Mt | − | C-probe | CPFV− | Bio-CCTCTGGGCTAATAGGACTACTTCTAATCTG-Spacer C3 | FV 1691 G-A | Mt | − | Target capture | Cpr9Fwd73_21 | GAGTCCTGCTACAAGATTTCA | FV 1691 G-A | Mt | − | RAM Fwd Primer | Cpr9Rvs87_21 | TGGACAGGCAAGGAATACAGG | FV 1691 G-A | Mt | − | RAM Rev Primer |
| Cpr10PTWt+ | GCTGAGAGTCACTTTTATTGGGAACCATAGAACTTCGTATCGCTCGC ACAAGAATCGTGTTTGAACAAGTAGGTGAATGGGCATTGAGGCTC | PT 20210 G-A | Wt | + | C-probe | CPPT+ | Bio-CTGCCCATGAATAGCACTGG-Spacer C3 | PT 20210 G-A | Wt | + | Target capture | Cpr10Fwd48_20 | TGCGAGCGATACGAAGTTCT | PT 20210 G-A | Wt | + | RAM Fwd Primer | Cpr10Rvs4_24 | GAGAGTCACTTTTATTGGGAACCA | PT 20210 G-A | Wt | + | RAM Rev Primer |
| Cpr12PTMt− | AGCCTCAATGCTCCCAGTGCTACGAGATAGACTGTGTCCTTCGGTG CGATGTTACCTTATGTGAATCACGATAAACTTCATTTGACTCTCAGC A | PT 20210 G-A | Mt | − | C-probe | CPPT− | Bio-CCGTGAAAGAATTATTTTTGTGTTTC-Spacer C3 | PT 20210 G-A | Mt | − | Target capture | Cpr12Fwd57_20 | AGGTAACATCGCACCGAAGG | PT 20210 G-A | Mt | − | RAM Fwd Primer | Cpr12Rev 5_20 | TCAATGCTCCCAGTGCTACG | PT 20210 G-A | Mt | − | RAM Rev Primer |
| Cpr13MTH677Wt− | CGATTTCATCATCACGCAGCTTTTCTTTGGTTATTGAAAGGTTCATC GCTTAGTGATACCCCGTCGCCAGCACTCGTCATCGCCTACCTGCGG GAGC | MTHFR 677 C-T | Wt | − | C-probe | CPMTH677− | Bio-TTTGAGGCTGACCTGAAGCAC-Spacer C3 | MTHFR 677 C-T | Wt | − | Target capture | Cpr13Fwd61_20 | GGGTATCACTAAGCGATGAA | MTHFR 677 C-T | Wt | − | RAM Fwd Primer | Cpr13Rev16_20 | GCAGCTTTTCTTTGGTTATT | MTHFR 677 C-T | Wt | − | RAM Rev Primer |
| Cpr14MTH677Mt+ | CTCCCGCAGACACCTTCTCCTTCCACGACGGAGGTATTGACTCGGG ATTTCCCAAAAGCAACAGATGGTGCCCCTATGATAGATGATGAAATC GA | MTHFR 677 C-T | Mt | + | C-probe | CPMTH677+ | Bio-GCGGAAGAATGTGTCAGCCTC-Spacer C3 | MTHFR 677 C-T | Mt | + | Target capture | Cpr14Fwd 56_20 | TTTGGGAAATCCCGAGTCAA | MTHFR 677 C-T | Mt | + | RAM Fwd Primer | Cpr14Rev 16_20 | TCTCCTTCCACGACGGAGGT | MTHFR 677 C-T | Mt | + | RAM Rev Primer |
| Cpr15MTH1298Wt− | AAGTGTCTTTGAAGTCTTCGTTCTTTACCTCACCAAGTCATCGTCCG CTCGTAGATAATGATAAGTGAGTAAAGTTATGAATGTTCTACTCCAG TGAAGA | MTHFR 1298 A-C | Wt | − | C-probe | CPMTH1298− | Bio-GAGGAGCTGCTGAAGATGTGG-Spacer C3 | MTHFR 1298 A-C | Wt | − | Target capture | Cpr15Fwd11_25 | GAAGTCTTCGTTCTTTACCTCACCA | MTHFR 1298 A-C | Wt | − | RAM Fwd Primer | Cpr15Rev64_24 | TTATCATTATCTACGAGCGGACGA | MTHFR 1298 A-C | Wt | − | RAM Rev Primer |
| Cpr16MTH1298Mt+ | CTTCACTGGTCAGCTCCTCCCCTACAGCAAAGCGGCGTCTGTGCGA AGATAATGATAAGTGAGTAAAGTTATGGATGGACGAAGAAAGACACT TG | MTHFR 1298 A-C | Mt | + | C-probe | CPMTH1298+ | Bio-ACCATTCCGGTTTGGTTCTCCC-Spacer C3 | MTHFR 1298 A-C | Mt | + | Target capture | Cpr16Fwd 7_20 | TGGTCAGCTCCTCCCCTACA | MTHFR 1298 A-C | Mt | + | RAM Fwd Primer | Cpr16Rev61_26 | CACTTATCATTATCTTCGCACAGACG | MTHFR 1298 A-C | Mt | + | RAM Rev Primer |
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