Effects of Growth Phase and Temperature on Activity within a Listeria monocytogenes Population: Evidence for RsbV-Independent Activation of at Refrigeration Temperatures
Figure 3
is activated in L. monocytogenes EGD-e wild-type in growth phase dependent manner at 4°C and 37°C. Flow cytometry analyses of WT-egfp strain were performed with BD AcurriC6 at the interval of three days (a) and 45 min (b) at 4°C and 37°C, respectively, and data was processed with BD CFlow Software. The numbers in % indicate the proportion of cells within population with fluorescence above the highest autofluorenscence observed for parent wT strain. Mean fluorescence intensity (MFI) values were shown for autofluorescence range and for EGFP gate separately. Each sample was performed in biological triplicate with duplicate analyses in each replication and a minimum of 100,000 events recorded for each sample.
