Early Appearance of Nonvisual and Circadian Markers in the Developing Inner Retinal Cells of Chicken
Figure 1
Immunocytochemistry for immunopurified RGC primary cultures at embryonic day 8 (E8) by Thy-1 (a), Opn4x (b) antibody purifications, and clock genes of RGC Opn4x cultures. (a) Primary cultures of embryonic RGCs purified by Thy-1 antibody immunopanning and maintained for 48–72 h were immunolabeled for DM1A, Opn4x, Thy-1, Rhod-4D2, and NeuN. (b) Immunocytochemistry for Opn4x- Prox-1, GABA, glutamine synthetase (GS), and NeuN, with DAPI staining in purified RGC cultures at E8 by Opn4x- antibody immunopurification. Primary cultures were visualized by confocal microscopy with specific primary antibodies as described in Section 2. Scale bar = 20 μm. (c) Primary cultures of RGC’s immunopurified by Opn4x-antibody immunolabeled with anti-Bmal1, Cry1, and Per2.4.
