Research Article
A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Viral and Bacterial Pathogens of Infectious Diarrhea
Table 1
Virus test panel for the evaluation of specificity of PCR primers.
| | Isolates | Number of the isolatesb | Targeted gene loci of virus | | VP6 | RDRPa | RDRP | ORF1a | Hexon | VP1 |
| | Rotavirus A | 9 | + | − | − | − | − | − | | Noroviruses G1 | 1 | − | + | − | − | − | − | | Noroviruses G2 | 6 | − | − | + | − | − | − | | Astrovirus | 2 | − | − | − | + | − | − | | Adenovirus | 10 | − | − | − | − | + | − | | Human bocavirus | 6 | − | − | − | − | − | + | | Coxsackie virus 16 | 2 | − | − | − | − | − | − | | Enterovirus 71 | 4 | − | − | − | − | − | − | | Influenza virus B | 3 | − | − | − | − | − | − | | Influenza virus A | 4 | − | − | − | − | − | − |
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RDRP, RNA-dependent RNA polymerase; ball the isolates in this panel were clinical samples.
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