A Novel Phospholipase A₂ (D49) from the Venom of the Crotalus oreganus abyssus (North American Grand Canyon Rattlesnake)

Table 2

Multiple alignment of fragment of CoaPLA₂.

		| -  -  -  Frag 1  -  - - -|    | - -  -  -  Frag 2  -  -  - - - - - | |  -  -  Frag 3
Fragments obtained		SLVQFEMLIMKVAKR  SGLFSYSAYGCYCGWGGHGR  PQDATDHCC
CoaPLA2	(1)	- - - - - - - - - - - - - - - -   SLVQFEMLIMKVAKR  SGLFSYSAYGCYCGWGGHGR  PQDATDHCC
Cvv_E6D	(1)	MRTLWIVAVLLLGVEG  SLVQFEMLIMKVAKR  SGLFSYSAYGCYCGWGGHGR  PQDATDHCC
Cvv_E6E	(1)	MRTLWILAVLLLGVEG  NLVQFELLIMKVAKR  SGLLSYSAYGCYCGWGGHGR  PQDATDRCC
Cvv_E6H	(1)	MRTLWIVAVLLLGVEG  SLVQFETLIMKIAGR  SGLLWYSAYGCYCGWGGHGL  PQDATDRCC
Cvv_E6G	(1)	MRTLWIVAVLLLGVEG  SLVQFEMMIIKVAKR  SGLFWYGAYGCYCGWGGQGR  PQDATDRCC
Cvv_E6A	(1)	MRTLWIVAVLLLGVEG  SLVQFETLIMKIAGR  SGLLWYSAYGCYCGWGGHGL  PQDATDRCC
Cadam	(1)	MRTLWIVAVLLLGVEG  SLVQFETLIMKVAKR  SGLLWYSAYGCYCGWGGHGR  PQDATDRCC
Chorrid	(1)	MRTLWIVAVLLLGVEG  SLVQFEMMIMEVAKR  SGLLWYSAYGCYCGWGGHGR  PQDATDRCC
Chhorr	(1)	MRTLWIVAVLLLGVEG  SLVQFEMMIMEVAKR  SGLLWYSAYGCYCGWGGHGR  PQDATDRCC
Scterg	(1)	MRTLWILAVLLLGVEG  NLIQFETLILKVAKK  SGMFSYSAYGCYCGWGGHGR  PQDATDRCC
Catrox	(1)	- - - - - - - - - - - - - - - -   SLVQFETLIMKIAGR  SGLLWYSAYGCYCGWGGHGL  PQDATDRCC
		- - - - - - - - |          | - - -  - - -  - - Frag 4 - -  - - -  - - |   | Frag 5 |  | Frag 6 |  |    Frag 7
Fragments obtained		FVHDCCYGK        TASYTYSEENGEIVCGGDDPCKK  QVCECDR  VAAICFR  DNIPTYD
CoaPLA2	(45)	FVHDCCYGK  - - - - - - -   TASYTYSEENGEIVCGGDDPCKK  QVCECDR  VAAICFR  DNIPTYD
Cvv_E6D	(61)	FVHDCCYGK  VTDCNPK  TASYTYSEENGEIVCGGDDPCKK  QVCECDR  VAAICFR  DNIPTYD
Cvv_E6E	(61)	FVHDCCYGK  VTDCNPK  TASYTYSEENGEIVCGGDDPCKK  QVCECDR  VAAICFR  DNIPSYD
Cvv_E6H	(61)	FVHDCCYGK  ATDCNPK  TVSYTYSVKNGEIICEDDDPCKR  QVCECDR  VAAVCFR  DNIPSYN
Cvv_E6G	(61)	FVHDCCYGK  ATDCNPK  TVSYTYSVKNGEIICEDDDPCKK  QVCECDR  VAAVCFR  DNIPSYN
Cvv_E6A	(61)	FVHDCCYGK  ATDCNPK  TVSYTYSEENGEIVCGGDNPCGT  QICECDK  AAAICFR  DNIPSYS
Cadam	(61)	FVHDCCYGK  ATNCNPK  TVSYTYSEENGEIVCGGDDPCGT  QICECDK  AAAICFR  DNIPSYD
Chorrid	(61)	FVHDCCYGK  ATDCNPK  RVSYTYSEENGEIVCGGDDPCGT  QICECDK  AAAICFR  DNIPSYD
Chhorr	(61)	FVHDCCYGK  ATDCNPK  RVSYTYSEENGEIVCGGDDPCGT  QICECDK  AAAICFR  DNIPSYD
Scterg	(61)	FVHDCCYGK  VTDCDPK  LDTYTYSEENGEIICGGDDPCKK  QICECDK  AAAICFR  DNKNTYN
Catrox	(45)	FVHDCCYGK  ATDCNPK  TVSYTYSEENGEIICGGDDPCGT  QICECDK  AAAICFR  DNIPSYD
		- - - -  |   | -  -  -  Frag 8  -  - |
Fragments obtained		NKYWR  FPPENCQEEPEPC
CoaPLA2	(98)	NKYWR  FPPENCQEEPEPC
Cvv_E6D	(121)	NKYWR  FPPENCQEEPEPC
Cvv_E6E	(121)	NKYIQ  FPAKNCQEKPEPC
Cvv_E6H	(121)	NNYKR  FPAENCREEPEPC
Cvv_E6G	(121)	NNYKR  FPAENCRGDPEPC
Cvv_E6A	(121)	NKYWL  FLPKNCRGDPEPC
Cadam	(121)	NKYWL  FPPKNCREEPEPC
Chorrid	(121)	NKYWL  FPPKNCQEEPEPC
Chhorr	(121)	NKYWL  FPPKNCREEPEPC
Scterg	(121)	NKYWR  LPTENCQEEPEPC
Catrox	(105)	NKYWL  FPPKDCREEPEPC

CoaPLA₂: Crotalus oreganuis abyssus; Cvv_E6D, Cvv_E6E, Cvv_E6H, Cvv_E6G, and Cvv_E6A (cDNA from Crotalus viridis viridis [37]), Cadam: Crotalus adamanteus [39]; Chorrid and Chhorrid: Croatus horridus and Crotalus horridus horridus [40]; Scterg: Sistrurus catenatus tergeminus [41]; Catrox: Crotalus atrox [42].