Research Article

Regulation of Melanopsins and Per1 by α-MSH and Melatonin in Photosensitive Xenopus laevis Melanophores

Figure 1

Quantitative PCR of Per1 in X. laevis melanophores after 2 medium changes in DD (a); 2 medium changes in LD 12 : 12 (b); 10−9 M α-MSH in DD (c); and 10−9 M melatonin in the photophase of LD 12 : 12 (d). Each bar represents the mean, ± SE, mRNA relative to the noted ZT. (a) Significantly different from (b); (c) significantly different from (d) by one-way ANOVA, Tukey’s post-test; means significantly different from the respective control at the same ZT, by two-way ANOVA and Bonferroni’s post-test. number shown on top of each bar in this and the following figures.
654710.fig.001a
(a) Medium changes
654710.fig.001b
(b) Medium changes in photophase
654710.fig.001c
(c) 10−9 M α-MSH
654710.fig.001d
(d) 10−9 M melatonin in photophase