Regulation of Melanopsins and Per1 by α-MSH and Melatonin in Photosensitive Xenopus laevis Melanophores
Figure 3
Quantitative PCR of Opn4m in X. laevis melanophores after to 2 medium changes in the photophase of LD 12 : 12 (a); 10−9 M melatonin in the photophase of LD 12 : 12 (b); 2 medium changes in the scotophase of LD 12 : 12 (c); 10−9 M melatonin in the scotophase of LD 12 : 12 (d); 2 medium changes in DD (e); 10−9 M α-MSH in DD (f). Each bar represents the mean, ± SE, mRNA relative to the noted ZT. (a) Significantly different from (b); (c) significantly different from (d), by one-way ANOVAandTukey’s post-test; means significantly different from the respective control at the same ZT, by two-way ANOVA and Bonferroni’s post-test.