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BioMed Research International
Volume 2014, Article ID 678301, 7 pages
Research Article

Emergent Bacteria in Cystic Fibrosis: In Vitro Biofilm Formation and Resilience under Variable Oxygen Conditions

1Institute for Biotechnology and Bioengineering (IBB), Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal
2LEPAE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal

Received 22 February 2014; Accepted 15 April 2014; Published 29 April 2014

Academic Editor: Andrei Surguchov

Copyright © 2014 Susana P. Lopes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1 shows a photograph of the mucoid physiology of I. limosus M53 strain used in this study, after growing onto TSA and incubated aerobically for 48 h. Figure S2 represents the content in protein and polysaccharides (in μg per cm2) for the matrix and cells of the biofilms of P. aeruginosa, I. limosus and D. pig rum developed under aerobic, microaerophilic and anaerobic conditions.The total proteins content for biofilm matrix and cells was measured with the BCA Protein Assay Kit (Bicinchoninic Acid, Thermo Scientific, Rockford, IL, USA), using bovine serum albumin as the standard. The total polysaccharides content was estimated according to the phenol-sulphuric acid procedure of Dubois et al. (1956), by using glucose as the standard.

  1. Supplementary Figures