Planarians Sense Simulated Microgravity and Hypergravity

Figure 3

Results of Experiment . Planarian trunks can regenerate properly in the RPM at 10°/s and under 4 g and 8 g LDC hypergravity. (a) Schedule of Experiment . (b) At day 5 all animals are regenerating properly (see the two black spots in the in vivo image and the brain stained with DAPI, in blue, and anti-synapsin, in green). At day 13 all animals are properly regenerated but RPM 60°/s, which were dead in 87,5% (see the eyes, the brain stained with anti-synapsin and the visual axons stained with anti-VC1). (c) The counting of pH3 positive cells in RPM and LDC planarians at days 5 and 12 of regeneration indicates that under hypergravity there is a significant decrease in the number of proliferative cells at 12 days of regeneration. Graphs error bars represent standard error of the mean. Data were analyzed by Student’s -test. Differences are considered significant at . Orange arrows indicate the regenerating eyes. Yellow arrows indicate the regenerating brain. White arrows indicate the regenerating eyes stained with anti-VC1. Yellow arrowhead points to the optic chiasm.