Cervical Cancer Cell Supernatants Induce a Phenotypic Switch from U937-Derived Macrophage-Activated M1 State into M2-Like Suppressor Phenotype with Change in Toll-Like Receptor Profile
Figure 1
Supernatant of HeLa, SiHa, and C-33A induces CD163 upregulation in U937-derived macrophages activated with lipopolysaccharides (LPS). U937 cells were differentiated into macrophages and treated with LPS for 24 h. Supernatants of cervical cancer (CC) cells were added to a final concentration of 30% in corresponding groups, and the cells were incubated for 7 days at 37°C in a humid atmosphere containing 5% CO2 and 95% air in Roswell Park Memorial Institute- (RPMI-) S medium. Then, the expression of CD163 was analyzed by flow cytometry (FC). Expression of CD163 in U937-derived macrophages activated with LPS and treated with supernatant of HeLa, SiHa, and C-33A cells during 3, 5, and 7 days (a). Percentage of CD163 expression in U937-derived macrophages activated with LPS and treated with supernatant of HeLa, SiHa, and C-33A during 7 days (b). Values of geometric mean fluorescence intensity (MFI) for CD163 expression in the different experimental groups (c). Dexamethasone (DEX) was used as positive control in the induction of CD163 expression. Results are shown as % and MFI and represent the mean ± standard deviation (SD) of three independent experiments performed in triplicate. Statistical analysis, Student’s -test. LPS versus Basal group. HeLa, SiHa, and C-33A groups versus Basal and LPS groups. DEX versus all groups.
