Cervical Cancer Cell Supernatants Induce a Phenotypic Switch from U937-Derived Macrophage-Activated M1 State into M2-Like Suppressor Phenotype with Change in Toll-Like Receptor Profile

Figure 4

Supernatant of cervical cancer (CC) cells induces a decrease in Nitric oxide (NO) in U937-derived macrophages activated with Lipopolysaccharides (LPS). U937-derived macrophages were activated with LPS and treated or not with supernatant of HeLa, SiHa, and C-33A cells or Dexamethasone (DEX) for 7 days. After culture, supernatant was collected. Quantification of NO in the form of Nitrate was determined in the supernatant of all experimental groups by colorimetric assay. The results represent average concentrations in μmol/L. Values represent mean ± standard deviations (SD). Statistical analysis, Student’s  -test. LPS and Basal versus HeLa, SiHa, and C-33A groups.