BioMed Research International / 2014 / Article / Fig 4

Research Article

Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom

Figure 4

Effect of BaTX-I, BaTX-II, and BaPLA2 on J774A.1 macrophage adhesion and detachment. For the adhesion test (a), 2 × 105 macrophages were incubated for 60 minutes with toxins (6 μg/mL) or RPMI (control) at 37°C in a humidified atmosphere of 5% CO2. For the detachment test (b), macrophages were incubated with RPMI for only 24 hours and then for 60 minutes with toxins (6 μg/mL) or RPMI (control) at 37°C in a humidified atmosphere of 5% CO2. Adhered cell levels were determined by optical density (550 nm) being proportional to the amount of incorporated Giemsa dye. Values represent the mean ± S.E.M. from 3 independent experiments.
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