Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom
Effect of BaTX-I, BaTX-II, and BaPLA2 on J774A.1 macrophage adhesion and detachment. For the adhesion test (a), 2 × 105 macrophages were incubated for 60 minutes with toxins (6 μg/mL) or RPMI (control) at 37°C in a humidified atmosphere of 5% CO2. For the detachment test (b), macrophages were incubated with RPMI for only 24 hours and then for 60 minutes with toxins (6 μg/mL) or RPMI (control) at 37°C in a humidified atmosphere of 5% CO2. Adhered cell levels were determined by optical density (550 nm) being proportional to the amount of incorporated Giemsa dye. Values represent the mean ± S.E.M. from 3 independent experiments.