Rho/ROCK Signal Cascade Mediates Asymmetric Dimethylarginine-Induced Vascular Smooth Muscle Cells Migration and Phenotype Change
Figure 3
ADMA changes the expression of differentiated markers via the activation of Rho/ROCK. Confluent VSMCs were pretreated with Y27632 (or not) at the concentration of 10 μM or overexpression of pAV-CMV-GFP-DDAH2 (or the blank vector of pAV-CMV-GFP) and then incubated with or without ADMA at the concentration of 10 μM for 12 h. The expression level of differentiated related markers of -actin and calponin was detected by Western Blotting. GAPDH was used as internal control. The graphs represent the relative expression level of every group by comparing to wild type VSMC without ADMA treatment. Data shown are the mean ± SEM from three independent experiments, ; .
