Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Chylomicron and VLDL Synthesis and Secretion in Caco-2 Cells
Figure 4
Incorporation of [l-14C]OA, [l-14C]EPA, and [l-14C]DHA into cell-associated and secreted lipids. Caco-2 cells plated on filter membranes and cultured for 21 days were incubated with [l-14C]OA, [l-14C]EPA, or [l-14C]EHA for 36 h. Cells were harvested and basolateral medium was collected for cell-associated (a) and secreted (b) lipids as described in Section 2. OA: oleic acid; EPA: eicosapentaenoic acid; DHA: docosahexaenoic acid; TG: triacylglycerol; PL: phospholipid; FFA: free fatty acids; CE: cholesteryl ester. Data are expressed as mean ± SD of 6 different experiments. Means without a common letter differ, .
