Research Article

Mitogenic Effects of Phosphatidylcholine Nanoparticles on MCF-7 Breast Cancer Cells

Figure 4

Proliferation of MCF-7 breast cancer cells incubated with phosphatidylcholine (PC) nanoparticles. Breast cancer cells were incubated for 48 hours with PC nanoparticle prepared in buffer pH 7.0 at 0.01 and 0.1% (w/v) or vehicle (Ct) in the absence of serum (a). Proliferation was determined by measuring BrdU incorporation with a BrdU ELISA. Triplicates were run for each treatment. Values were expressed in terms of percent of untreated control cells set as 100% (a). Data are expressed as the mean ± S.E.M. of the indicated number ( ) of independent experiments. Fold induction with respect to control was calculated (b). Statistical analysis was performed by ANOVA. Different letters denote significant difference at , whereas results with the same letter are not statistically different from each other.