Identification of a Novel Methylated Gene in Nasopharyngeal Carcinoma: TTC40

Table 1

Summary of primers used for MSP, bisulfite sequencing, and RT-PCR.

Primer sequences (5′-3′)	Annealing temperatures; (°C)	Product sizes; (bp)	PCR cycles
MSP “TTC40”
Stage-1 PCR
F-GAAGGTGGAGGGTGAGTT	52	439	25
R-AACCTAAAATCACCTTACTAACTCT
Stage-2 PCR
MF-ATTTTCGCGGAGTTGGCG	61	189	35
MR-CTCCGAAATCCGCGATACG
UF-GTTTTATTTTTGTGGAGTTGGTG	59	198	35
UR-CTCTCTCCAAAATCCACAATACA
Bisulfite sequencing “TTC40”
F-GTTGTTTTGGTGTTTTATTTT	50	294	35
R-AACCTAAAATCACCTTACTAACTCT
RT-PCR
“TTC40”
F-CCAGCCTTTCCCAAATCATA	61	187	40
R-ATCTGCCGGTATTTCTGTGG
“β-Actin”
F-GTCTCCCAAGTCCACACA	57	125	30
R-GCACGAAGGCTCATCATT