Research Article

Does Platelet-Rich Plasma Freeze-Thawing Influence Growth Factor Release and Their Effects on Chondrocytes and Synoviocytes?

Table 1

List of primers used in real-time PCR.

RNA templatePrimer sequences (5′–3′)Annealing temperature (°C)References*

GAPDH5′-TGGTATCGTGGAAGGACTCATGAC
3′-ATGCCAGTGAGCTTCCCGTTCAGC
60[17]

Collagen type II5′-GACAATCTGGCTCCCAAC
3′-ACAGTCTTGCCCCACTTAC
60PRIMER 3

Aggrecan5′-TCGAGGACAGCGAGGCC
3′-TCGAGGGTGTAGCGTGTAGAGA
60[18]

Sox-95′-GAG CAG ACG CAC ATC TC
3′-CCT GGG ATT GCC CCG A
60PRIMER 3

IL-1β5′-GTGGCAATGAGGATGACTTGTT
3′-TGGTGGTCGGAGATTCGTAG
60PRIMER 3

IL-65′-TAGTGAGGAACAAGCCAGAG
3′-GCGCAGAATGAGATGAGTTG
60PRIMER 3

IL-85′-CCAAACCTTTCCACCC
3′-ACTTCTCCACAACCCT
60PRIMER 3

IL-105′-CTTTAAGGGTTACCTGGGTTG
3′-CTTGATGTCTGGGTCTTGG
60PRIMER 3

TNF-α5′-AGCCCATGTTGTAGCAAACC
3′-ACCTGGGAGTAGATGAGGTA
60PRIMER 3

VEGF5′-TGATGATTCTGCCCTCCTC
3′-GCCTTGCCTTGCTGCTC
60PRIMER 3

FGF-β5′-CGGCTGTACTGCAAAAACGG
3′-TTGTAGCTTGATGTGAGGGTCG
60PRIMER 3

HGF5′-ATACTCTTGACCCTCACACC
3′-TGTAGCCTTCTCCTTGACCT
60PRIMER 3

TGF-β15′-CAACAATTCCTGGCGATACCT
3′-TAGTGAACCCGTTGATGTCC
60PRIMER 3

HAS-15′-TGGTGCTTCTCTCGCTCTACG
3′-GAACTTGGCAGGCAGGAGG
60[19]

HAS-25′-AAATGGGATGAATTCTTTGTTTATG
3′-GGCGGATGCACAGTAAGGAA
60[19]

HAS-35′-CAGCTGATCCAGGCAATCGT
3′-TGGCTGACCGGATTTCCTC
60[19]

TIMP-15′-CCGACCTCGTCATCAG
3′-GTTGTGGGACCTGTGGAA
60PRIMER 3

TIMP-35′-CCTTGGCTCGGGCTCATC
3′-GGATCACGATGTCGGAGTTG
60PRIMER 3

IL-45′-CAGTTCCACAGGCACAAG
3′-CTGGTTGGCTTCCTTCACA
60PRIMER 3

IL-135′-GCACACTTCTTCTTGGTC
3′-TGAGTCTCTGAACCCTTG
60PRIMER 3

IL-105′-CTTTAAGGGTTACCTGGGTTG
3′-CTTGATGTCTGGGTCTTGG
60PRIMER 3

MMP-135′-TCACGATGGCATTGCT
3′-GCCGGTGTAGGTGTAGA
60PRIMER 3

 *Primer sequences were obtained from published references and were indicated or designed using PRIMER 3.