ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury
Figure 4
ADAMTS proteoglycanases are expressed during SCI. (a) ADAMTS1 is expressed in spinal cord tissue of Adamts4−/− mice 1 and 7 days after injury, m = mature, asterisk = autocatalytic fragment. (b) ADAMTS9 is expressed in spinal cord tissue of Adamts4−/− mice in the sham-operated group 1 and 7 days after injury, z = zymogen (inactive), asterisks = uncharacterized fragments. (c) ADAMTS15 is expressed in spinal cord tissue of Adamts4−/− mice in the sham-operated group 1 and 7 days after injury, z = zymogen (inactive), asterisk = uncharacterized fragments. In all cases β-actin was used as a loading control (bottom panels). (d) Spinal cord injury triggered astrocyte accumulation (reactive astrocytes) at the injury site compared with sham-operated mice. Red = astrocyte, blue = nucleus. Scale bar = 50 μm.
