Research Article

Induction of Apoptosis in Human Multiple Myeloma Cell Lines by Ebselen via Enhancing the Endogenous Reactive Oxygen Species Production

Figure 3

The ROS production of MM cells treated with ebselen in presence or absence of NAC was determined by Singlet ROS Probe. Fluorescence images of Singlet ROS Probe loaded cells were obtained under laser confocal microscopy ((A) DMSO, (B) ebselen 40 μM, and (C) ebselen 40 μM + NAC 15 mM). The average of fluorescence intensity obtained under FACS shows that ebselen induced ROS increasing of MM cells in a concentration (b) and time (c) manner. Each value represents the mean ± SD from three independent experiments. *Significant difference compared to the control group ( , , one-way ANOVA with Scheffe’s test).
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(b)
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(c)