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BioMed Research International
Volume 2014, Article ID 702981, 8 pages
http://dx.doi.org/10.1155/2014/702981
Research Article

Caffeic Acid Phenethyl Ester Protects against Amphotericin B Induced Nephrotoxicity in Rat Model

1Department of Nephrology, School of Medicine, Internal Medicine, Süleyman Demirel University, East Campus, 32200 Isparta, Turkey
2Department of Medical Biology, School of Medicine, Mevlana University, 42030 Konya, Turkey
3Department of Dermatology, Isparta State Hospital, 32200 Isparta, Turkey
4Department of Biochemistry, School of Medicine, Süleyman Demirel University, 32200 Isparta, Turkey
5Department of Histology, School of Medicine, Süleyman Demirel University, 32200 Isparta, Turkey
6Department of Endocrinology and Metabolism, School of Medicine, Internal Medicine, Süleyman Demirel University, 32200 Isparta, Turkey

Received 28 February 2014; Accepted 31 May 2014; Published 16 June 2014

Academic Editor: Beatrice Charreau

Copyright © 2014 Atila Altuntaş et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study was conducted to investigate whether caffeic acid phenethyl ester (CAPE), an active component of propolis extract, has a protective effect on amphotericin B induced nephrotoxicity in rat models. Male Wistar-Albino rats were randomly divided into four groups: (I) control group (), (II) CAPE group () which received 10 μmol/kg CAPE intraperitoneally (i.p.), (III) amphotericin B group () which received one dose of 50 mg/kg amphotericin B, and (IV) amphotericin B plus CAPE group () which received 10 μmol/kg CAPE i.p. and one dose of 50 mg/kg amphotericin B. The left kidney was evaluated histopathologically for nephrotoxicity. Levels of malondialdehyde (MDA), nitric oxide (NO), enzyme activities including catalase (CAT), and superoxide dismutase (SOD) were measured in the right kidney. Histopathological damage was prominent in the amphotericin B group compared to controls, and the severity of damage was lowered by CAPE administration. The activity of SOD, MDA, and NO levels increased and catalase activity decreased in the amphotericin B group compared to the control group (, , , and , resp.). Amphotericin B plus CAPE treatment caused a significant decrease in MDA, NO levels, and SOD activity (, , and , resp.) and caused an increase in CAT activity compared with amphotericin B treatment alone (). CAPE treatment seems to be an effective adjuvant agent for the prevention of amphotericin B nephrotoxicity in rat models.