Figure 13: The transfection efficiency of lipoplexes. ARPE-19 cells were incubated with lipoplexes containing lipids C20-18, C20-20, or C30-20 against reference lipids DC-Chol and EPC with either Chol or DOPE as colipid at N/P molar ratio 0.5 : 1 for 4 h and transfection efficiencies, measured by GFP expression, determined by flow cytometry after 72 h. Statistical significance ( ) of 95% ( ).