Table 1: Size and surface charge (as measured by zeta potential, ZP) of C20-18, C20-20, C30-20, DC-Chol, and EPC lipoplexes in PBS at varying N/P molar charge ratios with either Chol or DOPE as colipids.

LipidColipid / ratiod H (nm)Mode (nm)PdlZP (mV)

DC-Chol0.5 : 111804770.718
Chol1 : 114804550.840
1.5 : 112205160.611
0.5 : 19004410.664
DOPE1 : 113106000.424
1.5 : 112905060.768*

EPC0.5 : 15104880.220
Chol1 : 13603800.058
1.5 : 13203220.234
0.5 : 114105280.750
DOPE1 : 19306250.366
1.5 : 12010*6560.862

C20-180.5 : 13202560.322
Chol1 : 13903610.285
1.5 : 11523*4900.702*
0.5 : 15504870.425
DOPE1 : 19104060.624
1.5 : 112704530.596

C20-200.5 : 15605480.130
Chol1 : 15705370.157
1.5 : 16605370.257
0.5 : 16404650.304
DOPE1 : 19705000.155
1.5 : 17505940.147

C30-200.5 : 1500355*†0.353
Chol1 : 18207630.178
1.5 : 17106970.260
0.5 : 11500937*0.144
DOPE1 : 19706050.419
1.5 : 111407680.220

Comparison between Chol and DOPE formulations of one lipid with the same molar charge ratio; Comparison between ratios (0.5 : 1 versus 1 : 1 or 1 : 1 versus 1.5 : 1) of the same lipid formulation.