Cationic Polyene Phospholipids as DNA Carriers for Ocular Gene Therapy
Table 1
Size and surface charge (as measured by zeta potential, ZP) of C20-18, C20-20, C30-20, DC-Chol, and EPC lipoplexes in PBS at varying N/P molar charge ratios with either Chol or DOPE as colipids.
Lipid
Colipid
/ ratio
d
H (nm)
Mode (nm)
Pdl
ZP (mV)
DC-Chol
0.5 : 1
1180
477
0.718
Chol
1 : 1
1480
455
0.840†
1.5 : 1
1220
516
0.611
0.5 : 1
900
441
0.664
DOPE
1 : 1
1310
600
0.424
1.5 : 1
1290
506
0.768*
EPC
0.5 : 1
510†
488
0.220†
Chol
1 : 1
360
380
0.058
1.5 : 1
320†
322†
0.234†
0.5 : 1
1410
528
0.750
DOPE
1 : 1
930
625
0.366
1.5 : 1
2010*
656
0.862
C20-18
0.5 : 1
320
256
0.322
Chol
1 : 1
390
361
0.285
1.5 : 1
1523*
490
0.702*
0.5 : 1
550
487
0.425
DOPE
1 : 1
910
406
0.624
1.5 : 1
1270
453
0.596
C20-20
0.5 : 1
560
548
0.130
Chol
1 : 1
570
537
0.157
1.5 : 1
660
537
0.257
0.5 : 1
640
465
0.304
DOPE
1 : 1
970
500
0.155
1.5 : 1
750
594
0.147
C30-20
0.5 : 1
500†
355*†
0.353
Chol
1 : 1
820
763
0.178
1.5 : 1
710
697
0.260
0.5 : 1
1500
937*
0.144
DOPE
1 : 1
970
605
0.419
1.5 : 1
1140
768
0.220
Comparison between Chol and DOPE formulations of one lipid with the same molar charge ratio; Comparison between ratios (0.5 : 1 versus 1 : 1 or 1 : 1 versus 1.5 : 1) of the same lipid formulation.