Abnormal Cardiac Autonomic Regulation in Mice Lacking ASIC3
Figure 3
Quantitative RT-PCR and Western blotting analyses of gene expression in sensory ganglia and heart. (a) The expression of Asic1a, Asic1b, Asic2b, and Asic3 is significantly lower in nodose ganglia (4- to 8-fold, *) than in DRG (C8 and T2), with the expression of Trpv1 significantly higher in nodose ganglia than in DRG (*). (b) The expression of TRPV1 did not significantly differ between Asic3 −/− and Asic3 +/+ mice in T2 DRG and nodose ganglia. (c) Gene expression of muscarinic acetylcholine receptors (M1, M2, and M4) and beta-adrenoceptors (adrb1, adrb2, and adrb3) not altered in hearts of Asic3 −/− mice. (d) Protein levels of adrenoceptors (b2-AR) and acetylcholine receptors (m2-AchR2) not altered in hearts of Asic3 −/− mice on western analysis. Expression of actin protein was used as an internal control. Asic3 +/+ mice and Asic3 −/− mice.
