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BioMed Research International
Volume 2014, Article ID 714173, 4 pages
http://dx.doi.org/10.1155/2014/714173
Research Article

Modified PEHPS Medium as an Alternative for the In Vitro Culture of Giardia lamblia

1Laboratorio de Bioquímica y Fisiología Celular, División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano Del Seguro Social, Administración de Correo No. 4, Apartado Postal 020-E Colonia Independencia, 64720 Monterrey, NL, Mexico
2Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Avenida Universidad s/n, Ciudad Universitaria, 66451 San Nicolás de los Garza, NL, Mexico
3Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Avenida Pedro de Alba y Manuel L. Barragán s/n, Ciudad Universitaria, 66451 San Nicolás de los Garza, NL, Mexico
4Coordinador Auxiliar Medico, Instituto Mexicano Del Seguro Social, Delegación Nuevo León, Gregorio Torres de Quevedo No. 1950 Ote, 64000 Monterrey, NL, Mexico

Received 28 February 2014; Revised 28 April 2014; Accepted 13 May 2014; Published 21 May 2014

Academic Editor: Thean Hock Tang

Copyright © 2014 Javier Vargas-Villarreal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Commercial culture media present interlot variations in biological activity. We have previously designed a homemade and economic culture medium, PEHPS medium, for the axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. Trophozoites of amoebae and trichomonads grow well in this medium. Furthermore, the medium is stable for several months when stored frozen or refrigerated. The objective of this work was to modify PEHPS medium to support the in vitro growth of Giardia lamblia. Inocula of 5 × 103 trophozoites/mL of G. lamblia were incubated at 36.5°C in modified PEHPS or TYI-S-33 medium. Then, the growths of the three Giardia strains in both media were compared. The logarithmic growth phase lasted 72 h; the mean yield of the strains ranged from 10.06 to 11.43 × 105Giardia trophozoites/mL, and the range of duplication time in the three strains was from 5.67 to 6.06 in modified PEHPS medium. These growth characteristics were not significantly different from those obtained with TYI-S-33 medium. We conclude that modified PEHPS medium might be used for the axenic cultivation of G. lamblia.