Adrenergic Activation of Melatonin Secretion in Ovine Pineal Explants in Short-Term Superfusion Culture Occurs via Protein Synthesis Independent and Dependent Phenomena
Figure 5
Experiment V. Effect of NE on N-acetylserotonin (a) and melatonin (b) release from the ovine pineal explants treated with transcription and translation inhibitors. The ovine pineal glands () were divided into three explants, which were randomly assigned to the experimental groups. The explants were incubated in the medium containing group I, 0.5% DMSO from 301 min to 360 min and 10 μM NE + 0.5% DMSO from 361 min to 720 min of the experiment; group II, 10 μM ACT D from 301 min to 360 min and 10 μM NE + 10 μM ACT D from 361 min to 720 min of the experiment; and group III, 100 μM CLX from 301 min to 360 min and 10 μM NE + 100 μM CLX from 361 min to 720 min of the experiment. The data presented are means. The values significantly different () between groups were signed with different letters. Black line is the level of NE in the culture medium. Blue bar is period of incubation in the medium with inhibitors; red bar is period of incubation in the medium with NE.
