Vascular Endothelial Growth Factor Increases during Blood-Brain Barrier-Enhanced Permeability Caused by Phoneutria nigriventer Spider Venom
Figure 6
CaB and GAD immunohistochemistry in the cerebellar cortex of rats aged 14 days after i.p. injection of saline solution ((a) and (e)) or P. nigriventer venom PNV ((b) and (f)). (a) and (b) show CaB labeling (24 hours after saline or PNV exposure, resp.) in Purkinje cell bodies, including nuclei (arrows) and processes crossing the molecular layer (ML). (c) and (d) show immunoblots and representative histograms of the densitometric CaB values of rats injected with saline (A) or PNV (C) at different time points. (e) and (f) show GAD labeling in Purkinje cell bodies and cell processes within the molecular layer (arrow). The physiologic GAD labeling was weak in controls (e) whereas it was strong in envenomed animals (f). (g) and (h) show immunoblots and representative histograms of the densitometric GAD values of rats injected with saline (E) or PNV (G) at different time points (/time). The membranes were stripped and reprobed to β-actin, confirming equal protein loading in the gel ((B), (D), (F), and (H)). Values are mean ± SEM; unpaired Student t-test. ; ; compared to control at each time point; ; ; compared to corresponding adults at the same time interval. P = pia mater; PC = Purkinje cells; ML = molecular layer; GL = granular layer. Bars = 25 μm for all panels.
