Ginseng Is Useful to Enhance Cardiac Contractility in Animals
Figure 4
Effects of ginseng on intracellular calcium and TnI phosphorylation in neonatal rat cardiomyocytes. Changes in intracellular calcium were detected with fura-2 by using a fluorescence spectrofluorometer. The neonatal rat cardiomyocytes were placed in buffered physiological saline solution with 5 μM of fura-2-AM and incubated for 1 h. After recording the baseline value, ginseng was added into the cuvette with/without GSK0660 to detect the free intracellular calcium (a). Effects of ginseng on PPARδ expression (b) and TnI phosphorylation (c) in the neonatal rat cardiomyocytes were indicated. Cells treated with ginseng for 24 hours were harvested to measure the protein level of PPARδ expression and TnI phosphorylation using Western blotting analysis. All values are presented as mean ± SEM (). compared with the control group.
