Hyaluronan-Phosphatidylethanolamine Polymers Form Pericellular Coats on Keratinocytes and Promote Basal Keratinocyte Proliferation
Figure 4
Topical A647-HA-PE enters the epidermis. Significantly increased fluorescent staining of A647-HA-PE is observed compared to A647-HA in the epidermis (stratum basale to stratum granulosum) at all time points up to 24 h (mean ± S.E.M, 20 skin images) (∗, ∗∗) (right panel). Representative confocal images of A647-HA-PE and A647-HA treated mice sectioned at 24 h are shown. Merged Dapi and Alexa647 staining are shown to indicate cellularity in tissue sections. The dashed line indicates the junction of the epidermis and dermis. Arrowheads indicate areas of enhanced pericellular fluorescence. A647-HA-PE and A647-HA staining was pseudocolored green. Blue staining is DAPI. An untreated adjacent skin section (no treatment) photographed using the Alexa647 channel is shown as a control for autofluorescence.
