Amarogentin, a Secoiridoid Glycoside, Abrogates Platelet Activation through PLCγ2-PKC and MAPK Pathways

Figure 3

Effects of amarogentin on the phosphorylation of MAPK and Akt induced by collagen in human platelets. Washed platelets (1.2 × 10⁹ cells/mL) were incubated with solvent control (0.5% DMSO) or amarogentin (15 μM~60 μM) and then treated with 1 μg/mL collagen to induce platelet activation. The subcellular extracts were analyzed for the phosphorylation of p38 (a), ERK2 (b), JNK1 (c), and Akt (d) by western blotting. Data are presented as the mean ± SEM (𝑛=3). ^∗∗∗𝑃<0.001, compared with the solvent control group (resting); ^#𝑃<0.05 and ^##𝑃<0.01, compared with the positive control group (collagen only).

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