Description of a Novel Adhesin of Mycobacterium avium Subsp. paratuberculosis

Table 3

Reactivity of bovine sera to the protein EF-Tu by line print immunoassay. 20 μL of antigens was applied onto a nitrocellulose membrane and simultaneously confronted to sera from 10 healthy animals, 8 animals with bovine tuberculosis (TBB), and 25 animals with paratuberculosis (PTB). Sixty four percent of MAP positive sera recognized EF-Tu.

Number of sera with positive recognition
Antigen	Healthy (𝑛=10)	PTB infected (𝑛=25)	TBB infected (𝑛=8)
PPDA	4	16	3
PPA-3	1	18	2
PPDB	6	1	4
EF-Tu	8	16	7