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BioMed Research International
Volume 2014 (2014), Article ID 731017, 3 pages

Erratum to “Use of Metarhizium anisopliae Chitinase Genes for Genotyping and Virulence Characterization”

International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi 00100, Kenya

Received 3 September 2013; Accepted 11 September 2013; Published 20 January 2014

Copyright © 2014 Saliou Niassy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Errors occurred during the uploading of the reviewed article and the authors would like to add some additional information. The following changes are to be considered to improve the quality of the paper.

Main Text. Page 4, Section 3.4 should be changed to “Comparison of the chi2 nucleotide sequences from all selected M. anisopliae (GenBank Accession (GBA): KF445078-85) isolates originating from three different parts of Africa showed no differences in the open reading frames composed of 229 amino acid residues. However, when compared with the similar chitinase sequences retrieved from NCBI database, there were differences in amino acid composition (Figure 2).”

Figure 2: The multiple sequence alignment (MAFFT; geneious 6.1.6 software) showing the relationship between the ICIPE Metarhizium anisopliae isolates' Chitinase 2 sequences and similar sequences obtained from NCBI. The highlighted residues in red (VI and YR) show the conserved residues of CID.

Page 4, Section 3.4 should be changed to “The phylogenic analysis showed over 95% amino acid identity of chitinase chi2 sequence. Metarhizium anisopliae var. acridum strain CQMa 102 (GBA: EFY85519) and M. robertsii ARSEF 23 (GBA: EFY95562) were genetically different from other M. anisopliae including the icipe chi2 consensus and the other three outgroups M34412 (GBA: ACU30524), E6 (GBA: AAY34347), and ARSEF 7524 (GBA: ACU30523) (Figure 3).”

Figure 3: A dendrogram showing the relationships between the chi2 gene of icipe isolates and the related sequences of the outgroup isolates.

Page 4, Section 3.6. should be changed to “All M. anisopliae var. anisopliae ICIPE isolates had identical chi4 nucleotide sequences (JX898505-12). After the editing process to remove the ambiguous base calls a BLAST analysis using chi4 sequence on NCBI GenBank database revealed the highest amino acid identities to M. anisopliae var. anisopliae M34412 (GBA: ACU30522) and ARSEF7524 (GBA: ACU30521) (Figure 5).”

Figure and Legands. The legands of Figures 25 were changed as shown below.

Figure 4: Chitinase2 (chi2) model as predicted using the Swiss-PdB Viewer. The residues highlighted (Val238 and Ile239; Tyr325 and Arg326) represent conserved residues in the Carbohydrate Insertion Domain (CID) of chitinases.
Figure 5: The multiple sequence alignment (MAFFT; Geneious 6.1.6 software) showing the relationship between the ICIPE Metarhizium anisopliae isolates’ Chitinase4 sequences and similar sequences obtained from NCBI as well as IMI330189 (GBA: JX898513).