Detection Progress of Selected Drugs in TLC

Table 1

Fundamental absorption band of drugs, color of spot of detected drugs, background color of chromatogram, limit of detection, and linearity range.

Detected drug	Stationary phase	Detection way	Fundamental absorption band of drug 𝜆max (nm)	Color of spot of detected drug	Background color of chromatogram	Limit of detection (𝜇g)	Linearity range (𝜇g spot^−1) (r, correlation coefficient)	Reference
Estradiol	Neutral aluminum oxide 60F254  toluene + ethyl acetate (1 : 1, v/v)	Without using visualizing reagent	205	Lack of colored spot in visible light	White	0.32	1.10÷25.00  (𝑟=0.9951)	[43]
Estradiol		Barton’s reagent	233	Dark blue/green	Willow-green	0.32	0.53÷10.24  (𝑟=0.9975)	[43]
Estradiol		Sulphuric acid	278	Light orange	Light beige	0.10	0.88÷12.80  (𝑟=0.9974)	[43]
Estradiol		Gentian violet	226	Violet/blue	Violet	0.32	0.53÷25.00  (𝑟=0.9943)	[43]
Estradiol		Methylene violet	227	Grey/blue	Light grey	0.32	0.88÷3.36  (𝑟=0.9975)	[43]
Estradiol	Neutral aluminum oxide 150F254 toluene + ethyl acetate (1 : 1, v/v)	Without using visualizing reagent	207	Lack of colored spot in visible light	White	0.32	1.37÷20.00  (𝑟=0.9972)	[43]
Estradiol		Gentian violet	598	Dark violet	Violet	0.32	0.88÷20.00  (𝑟=0.9879)	[43]
Estradiol		Methylene violet	204	Grey/blue	Light grey	0.53	1.37÷25.00  (𝑟=0.9983)	[43]
Salicylanilide	Silica gel 60F254 (#1.05744) chloroform	Without using visualizing reagent	307	Lack of colored spot in visible light	White	0.07	5.00÷30.00  (𝑟=0.9802)	[44]
Salicylanilide		Brilliant green	597	Dark green	Green	0.70	3.00÷30.00  (𝑟=0.9929)	[44]
(±)-𝛼-Tocopherol	Silica gel 60 toluene	Without using visualizing reagent	270	Lack of colored spot in visible light	White	0.45	3.46÷20.00  (𝑟=0.9894)	[41]
(±)-𝛼-Tocopherol		2,2′-Bipyridine-iron (III) chloride	524	Raspberry red	Light beige	2.08	5.76÷30.00  (𝑟=0.9927)	[41]
(±)-𝛼-Tocopherol		Methylene violet	270	Azure with white border	Light grey-blue	0.30	0.75÷5.76  (𝑟=0.9949)	[41]
(±)-𝛼-Tocopherol		Methyl green	270	Light green	Green	0.30	0.75÷5.76  (𝑟=0.9863)	[41]
(+)-𝛼-Tocopherol acetate	Silica gel 60 toluene	Without using visualizing reagent	203	Lack of colored spot in visible light	White	0.30	0.75÷20.00  (𝑟=0.9978)	[41]
(+)-𝛼-Tocopherol acetate		Rhodamine B	203	Dark pink	Pink	0.45	1.25÷16.00  (𝑟=0.9936)	[41]
(+)-𝛼-Tocopherol acetate		Gentian violet	203	Dark violet	Violet	0.75	2.08÷25.00 (𝑟=0.9908)	[41]
(+)-𝛼-Tocopherol acetate		Methyl green	203	Green (small contrast with background color)	Green	0.75	1.25÷25.00  (𝑟=0.9943)	[41]
(+)-𝛼-Tocopherol acetate		Janus blue	203	Dark blue	Blue	0.75	1.25÷25.00  (𝑟=0.9938)	[41]
Ibuprofen	Silica gel 60F254  chloroform + methanol (50 : 1.4, v/v)	Without using visualizing reagent	200	Lack of colored spot in visible light	White	0.62	1.25÷10.00  (𝑟=0.9958)	[42]
Ibuprofen		Methylene violet	200	White-blue with dark blue border	Light	1.25	5.00÷25.00  (𝑟=0.99549)	[42]
Stearic acid	Silica gel 60 n-hexane + acetone (4 : 1, v/v)	Rhodamine B	585	Dark pink	Pink	1.25	1.56÷28.40  (𝑟=0.9913)	[45]
Stearic acid		Methylene blue	687	Blue with light border	Blue	0.07	0.41÷7.44  (𝑟=0.9975)	[45]
Stearic acid		Janus blue	617	Navy blue with white border	Blue	0.12	1.56÷7.44  (𝑟=0.9928)	[45]
Stearyl alcohol	Silica gel 60 n-hexane + ethyl acetate + methanol (46 : 3.5 : 0.5, v/v/v)	Rhodamine B	586	Dark pink	Pink	1.48	2.90÷27.00  (𝑟=0.9964)	[45]
Stearyl alcohol	Silica gel 60 n-hexane + ethyl acetate + methanol (46 : 3.5 : 0.5, v/v/v)	Malachite green	644	Green with light border	Green	0.32	0.89÷8.85 (𝑟=0.9959)	[45]
Stearyl alcohol	Silica gel 60 n-hexane + ethyl acetate + methanol (46 : 3.5 : 0.5, v/v/v)	Janus blue	655	Navy blue	Blue	0.32	0.53÷7.08 (𝑟=0.9995)	[45]