Review Article

Detection Progress of Selected Drugs in TLC

Table 2

Characteristics of densitometric band of detected drugs, modified contrast index, densitometric visualizing index, modified broadening index, and detection index.

Detected drug Stationary phase, mobile phase Detection way Densitometric band characteristic of detected drug Modified contrast index (AU/°)Densitometric visualizing index (Au/ g·°) Modified broadening index ( g/AU)Detection index ( g/AU)Reference
Area (AU)Height (AU)α (°)

EstradiolNeutral aluminum oxide 60F254 toluene + ethyl acetate (1 : 1, v/v)Without using visualizing reagent1304824614.516.9661.9160.32/495[43]
EstradiolBarton’s reagent237862551319.6151.0510.32/1070[43]
EstradiolSulphuric acid 447094971338.2310.5590.10/850[43]
EstradiolGentian violet113482201514.6672.2030.32/760[43]
EstradiolMethylene violet136222211713.0001.8350.32/705[43]

EstradiolNeutral aluminum oxide 150F254 toluene + ethyl acetate (1 : 1, v/v)Without using visualizing reagent114241921314.7692.1880.32/409[43]
EstradiolGentian violet10906127207.7002.2920.32/796[43]
EstradiolMethylene violet8466154334.6672.9530.53/490[43]

SalicylanilideSilica gel 60F254 (#1.05744) chloroformWithout using visualizing reagent72889707978.511.5700.6860.07/1590[44]
SalicylanilideBrilliant green91842704888.01.6400.5440.70/11112[44]

(±)- -Tocopherol Silica gel 60 tolueneWithout using visualizing reagent345194591628.690.4790.7240.45/3100[41]
(±)- -Tocopherol2,2′-Bipyridine-iron (III) chloride60474698977.560.3230.4132.08/8915[41]
(±)- -TocopherolMethylene violet40426636879.501.6840.6180.30/3456[41]
(±)- -TocopherolMethyl green37196576964.001.3780.6720.30/1564[41]

(+)- -Tocopherol acetate Silica gel 60 tolueneWithout using visualizing reagent337965911153.731.0240.7400.30/1100[41]
(+)- -Tocopherol acetateRhodamine B260314551530.330.3860.9600.45/1511[41]
(+)- -Tocopherol acetateGentian violet294415451054.500.3920.8490.75/1884[41]
(+)- -Tocopherol acetateMethyl green302265641151.270.3660.8270.75/3911[41]
(+)- -Tocopherol acetateJanus blue301255651151.360.3650.8300.75/3750[41]

IbuprofenSilica gel 60F254Without using visualizing reagent556444452617.1150.3420.4490.62/3325[42]
Ibuprofenchloroform + methanol (50 : 1.4, v/v)Methylene violet53696348359.9430.1230.4661.25/4438[42]

Stearic acid Silica gel 60 n-hexane + acetone (4 : 1, v/v)Rhodamine B656782312.6450.0174.321.25/900[45]
Stearic acidMethylene blue309183681426.2863.1550.920.07/245[45]
Stearic acidJanus blue361754621142.0002.7410.790.12/604[45]

Stearyl alcohol Silica gel 60 n-hexane + ethyl acetate + methanol (46 : 3.5 : 0.5, v/v/v)Rhodamine B3605100137.6920.0197.491.48/234[45]
Stearyl alcoholMalachite green12378333747.5710.5532.180.32/382[45]
Stearyl alcoholJanus blue109632691320.6920.2642.460.32/214[45]