BioMed Research International / 2014 / Article / Tab 2 / Review Article
Detection Progress of Selected Drugs in TLC Table 2 Characteristics of densitometric band of detected drugs, modified contrast index, densitometric visualizing index, modified broadening index, and detection index.
Detected drug
Stationary phase, mobile phase
Detection way
Densitometric band characteristic of detected drug
Modified contrast index (AU/°) Densitometric visualizing index (Au/
g·°)
Modified broadening index (
g/AU) Detection index (
g/AU) Reference Area (AU) Height (AU) α (°)Estradiol Neutral aluminum oxide 60F254 toluene + ethyl acetate (1 : 1, v/v) Without using visualizing reagent 13048 246 14.5 16.966 — 1.916 0.32/495 [43 ] Estradiol Barton’s reagent 23786 255 13 19.615 — 1.051 0.32/1070 [43 ] Estradiol Sulphuric acid 44709 497 13 38.231 — 0.559 0.10/850 [43 ] Estradiol Gentian violet 11348 220 15 14.667 — 2.203 0.32/760 [43 ] Estradiol Methylene violet 13622 221 17 13.000 — 1.835 0.32/705 [43 ] Estradiol Neutral aluminum oxide 150F254 toluene + ethyl acetate (1 : 1, v/v) Without using visualizing reagent 11424 192 13 14.769 — 2.188 0.32/409 [43 ] Estradiol Gentian violet 10906 127 20 7.700 — 2.292 0.32/796 [43 ] Estradiol Methylene violet 8466 154 33 4.667 — 2.953 0.53/490 [43 ] Salicylanilide Silica gel 60F254 (#1.05744) chloroform Without using visualizing reagent 72889 707 9 78.5 11.570 0.686 0.07/1590 [44 ] Salicylanilide Brilliant green 91842 704 8 88.0 1.640 0.544 0.70/11112 [44 ] (±)-
-Tocopherol
Silica gel 60
toluene Without using visualizing reagent 34519 459 16 28.69 0.479 0.724 0.45/3100 [41 ] (±)-
-Tocopherol 2,2′-Bipyridine-iron (III) chloride 60474 698 9 77.56 0.323 0.413 2.08/8915 [41 ] (±)-
-Tocopherol Methylene violet 40426 636 8 79.50 1.684 0.618 0.30/3456 [41 ] (±)-
-Tocopherol Methyl green 37196 576 9 64.00 1.378 0.672 0.30/1564 [41 ] (+)-
-Tocopherol acetate
Silica gel 60
toluene Without using visualizing reagent 33796 591 11 53.73 1.024 0.740 0.30/1100 [41 ] (+)-
-Tocopherol acetate Rhodamine B 26031 455 15 30.33 0.386 0.960 0.45/1511 [41 ] (+)-
-Tocopherol acetate Gentian violet 29441 545 10 54.50 0.392 0.849 0.75/1884 [41 ] (+)-
-Tocopherol acetate Methyl green 30226 564 11 51.27 0.366 0.827 0.75/3911 [41 ] (+)-
-Tocopherol acetate Janus blue 30125 565 11 51.36 0.365 0.830 0.75/3750 [41 ] Ibuprofen Silica gel 60F254 Without using visualizing reagent 55644 445 26 17.115 0.342 0.449 0.62/3325 [42 ] Ibuprofen chloroform + methanol (50 : 1.4, v/v) Methylene violet 53696 348 35 9.943 0.123 0.466 1.25/4438 [42 ] Stearic acid
Silica gel 60 n-hexane + acetone (4 : 1, v/v) Rhodamine B 6567 82 31 2.645 0.017 4.32 1.25/900 [45 ] Stearic acid Methylene blue 30918 368 14 26.286 3.155 0.92 0.07/245 [45 ] Stearic acid Janus blue 36175 462 11 42.000 2.741 0.79 0.12/604 [45 ] Stearyl alcohol
Silica gel 60 n-hexane + ethyl acetate + methanol (46 : 3.5 : 0.5, v/v/v) Rhodamine B 3605 100 13 7.692 0.019 7.49 1.48/234 [45 ] Stearyl alcohol Malachite green 12378 333 7 47.571 0.553 2.18 0.32/382 [45 ] Stearyl alcohol Janus blue 10963 269 13 20.692 0.264 2.46 0.32/214 [45 ]