732516.fig.003a
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732516.fig.003b
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Figure 3: Baicalein induces apoptosis in HCC cells. (a) Morphology of SMMC-7721 and Bel-7402 cells under contrast microscopy (40x) after treating with 0, 100, or 200 μM of Baicalein for 24 h. (b and c) The protein levels of full length and cleaved form of caspase-9, caspase-3, and PARP in SMMC-7721 (b) and Bel-7402 (c) cells were determined by western blotting following the treatment of the indicated dose of baicalein for the indicated time. GAPDH served as a loading control. (d) Morphology of nuclei after treatment of the indicated dose of baicalein for 24 h. Pyknosis and karyorrhexis were pointed by white arrow.