732516.fig.007a
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732516.fig.007b
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Figure 7: Baicalein induces protective autophagy. (a) HCC cells were treated with the indicated dose of baicalein for the indicated time and the level of LC-3 was determined. (b) SMMC-7721 cells were transfected with scrambled RNA (NC) or Atg5-targeting siRNA (si-Atg5) for 48 h and then treated with 0, 100, and 200 μM baicalein for another 24 h. Cleaved PARP and Atg5 were analyzed by western blotting. (c) SMMC-7721 cells were transfected with scrambled RNA (NC) or Beclin 1-targeting siRNA (si-Beclin 1) for 48 h and incubated with the indicated concentration of baicalein for 24 h. Cleaved PARP and Beclin 1 were analyzed by western blotting. GAPDH served as a loading control.