Research Article

Silencing of Plasma Membrane Ca2+-ATPase Isoforms 2 and 3 Impairs Energy Metabolism in Differentiating PC12 Cells

Figure 2

Microscopic characteristic of differentiated PC12 lines. (a) Morphological changes induced by differentiation with 1 mM db-cAMP. The morphology was analyzed with an Olympus CK-40 inverted microscope and images were captured using a CCD camera. Scale bar 10 μm. (b) Evaluation of mitochondrial mass with MitoTracker Green TM in fixed cells using TCS S5 confocal microscope. Scale bar 20 μm. (c) Representative micrographs of cell ultrastructure at 105.000x magnification with mitochondria marked with asterisks. Control: mock-transfected PC12, _2: PMCA2-reduced PC12, and _3: PMCA3-reduced PC12.
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