Silencing of Plasma Membrane Ca2+-ATPase Isoforms 2 and 3 Impairs Energy Metabolism in Differentiating PC12 Cells
Figure 4
Flow cytometry analysis of differentiated PC12 cells after drug treatment. (a) The analysis was performed using Annexin V-FITC/propidium iodide staining (FACScan, Becton Dickinson). Typical dot plots are presented ( independent experiments with similar results) and indicate : necrotic cells, : live cells, LA: late apoptosis, and EA: early apoptosis. (b) Quantification of apoptosis and necrosis after drug treatment. The bars indicate the distribution (% ± SD) within cell population. Data shown are the mean of independent experiments. Quantification of cells was done using CellQuest software (Becton Dickinson). versus corresponding untreated cells; versus treated control line as indicated. : mock-transfected PC12, _2: PMCA2-reduced PC12, and _3: PMCA3-reduced PC12.
